Radioimmunoassay of Triiodothyronine

Triiodothyronine radioimmunoassay.

Highly specific antisera to triiodothyronine (T(3)) were prepared by immunization of rabbits with T(3)-bovine serum albumin conjugates. Antisera with T(3) binding capacity of up to 600 ng/ml were obtained. The ability of various thyronine derivatives to inhibit the binding of T(3-) (125)I to anti-T(3) serum was found to vary considerably. l-T(3), d-T(3) and several triiodoanalogues were potent ...

Rapid radioimmunoassay of triiodothyronine.

The method used in this report is based on the immunological production of a specific antisera to tnlodothyronine (T3) in rabbits. Conducted at 4 #{176}C, the radioimmunoassay requires three to five days to complete. Hoping to speed this assay, we investigated the influence of time, temperature, and pH. Incubation at 37 #{176}C and pH 8.0 for 30 mm did not deleteriously affect either precision,...

Radioimmunoassay of Triiodothyronine TOSHIRO SAKURADA

SAKURADA, T., SAITO, S., YAMAGUCHI, T., YAMAMOTO , M., DEMURA, R., DEMURA, H., FUKUGHI, S., YOSHIDA, K. and TORIKAI, T. Radioinonunoassay of Triiodothyronine. Tohoku J. exp. Med ., 1973, 110 (4), 329-340 Immuniza tion of rabbits with triiodothyronine-methyl ester hydrochloride conjugated to bovine serum albumin resulted in the production of antibody against triiodothyro nine. Using dextran-coat...

Radioimmunoassay of triiodothyronine in unextracted human serum.

Serum triiodothyronine (T-3) concentrations have been estimated by radioimmunoassay using unextracted serum. The serum T-3 concentrations have been shown to be similar in two separate European populations (0.76-1.67 ng/ml). Raised T-3 values have been observed in all subjects with hyperthyroidism. Low values are seen in hypothyroidism although there is some overlap with the normal range. There ...

The radioimmunoassay of triiodothyronine and its clinical application.